Program and book of abstracts 1st conference

October 17 th – 20 th , 2022 Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia Book of Abstracts Program & 1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine”

www.euh2academy.org • secretary@euh2academy.org

INTERCEDU International Center of Education

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“ Organizer

www.euh2academy.org • secretary@euh2academy.org European Academy for Molecular Hydrogen Research in Biomedicine

Coorganizers

INSTITUTE FOR HEART RESEARCH OF SLOVAK ACADEMY OF SCIENCES www.usrd.sav.sk/en CENTRE OF EXPERIMENTAL MEDICINE OF SLOVAK ACADEMY OF SCIENCES www.cem.sav.sk/en/

SLOVAK ACADEMY OF SCIENCES www.sav.sk

INTERNATIONALACADEMYOFCARDIOVASCULARSCIENCES www.iacs.sav.sk

MINISTRYOF EDUCATION, SCIENCE, RESEARCH AND SPORT OF THE SLOVAK REPUBLIC www.minedu.sk

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine”

October 17 th – 20 th , 2022 Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia Book of Abstracts Program &

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Welcome On behalf of the Organizing and Program Committee we are pleased to welcome you to „1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine“ held on October 17 th – 20 th , 2022 in Congress Centre of Slovak Academy of Science, Smolenice Castle, Slovakia organized by Institute for Heart Research, Centre of Experimental Medicine, Slovak Academy of Sciences in collaboration with International Academy of Cardiovascular Sciences, International Society for Adaptive Medicine, Ministry of Education, Science, Research and Sport of the Slovak Republic, and other institutions in Slovakia. The theme of the meeting is „Hydrogen in Biomedicine“. The goal of this scientific meeting is to bring together the top scientists, research fellows and trainees, from all over the world to participate in this scientific forum including from Canada, USA, Iran, East Jerusalem, Slovakia, Czechia, China, Japan, Russia, Ukraine, United Kingdom, France, Germany, Republic of Serbia, Republic of Korea, and India. The meeting will be comprised of and 10 scientific sessions and will focus on pluripotent effect of Molecular Hydrogen on health, cardio protection, cardiometabolic diseases, oxidative stress molecular genetics, prevention, regeneration, heart failure, nutrition, ageing, COVID-19 treatment, applied aspects in cardiovascular health care delivery and many other biological effects. The scientific program committee did an outstanding job in assembling a stellar line up of expert speakers and integrative aspects of cardiovascular medicine with a special focus on early and midcareer investigators. A highlight of the meeting is the poster session and plenary symposium, landmark lectures, and awards programs. We are particularly proud of the large attendance of early and mid-career scientists. The combination of outstanding speakers, themed symposia and meeting venue will offer a great scientific program and enjoyable atmosphere. We hope that despite the tight scientific schedule there will be sufficient space for fruitful and stimulating discussions and chances to enjoy the beautiful nature of the Small Carpathian Mountins and cultural spirit of Slovakia. We would like to thank you for attending the conference and wish you a warm welcome to Smolenice Castle, Slovakia.

RNDr. Branislav Kura, Ph.D. Chair of the Meeting branislav.kura@savba.sk

Prof. Ján Slezák, MD, DSc., FIACS Honorary Chairman of the Meeting jan.slezak@savba.sk

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia Organizers

Slezak Jan (Slovakia) Kura Branislav (Slovakia) Kalocayova Barbora (Slovakia) Szeiffova Bacova Barbara (Slovakia) Sykora Matus (Slovakia) Sagatova Andrea (Slovakia)

Fulop Marko (Slovakia) Botek Michal (Czechia)

Hancock John T. (United Kingdom) Ostojic Sergej M. (Republic of Serbia) Medvedev Oleg Stephanovich (Russian Federation)

Andelova Katarina (Slovakia) Ferenczyova Kristina (Slovakia)

International Advisory Board

Artamonov Mikhail (U.S.A.) Cinquin Philippe (France) Dhalla Naranjan S. (Canada) Lahham Adnan (East Jerusalem) LeBaron Tyler W. (U.S.A.) Lee Kyu-Jae (Republic of Korea)

Liu Jiankang (China) Luby Stefan (Slovakia) Noda Mami (Japan) Ohta Shigeo (Japan)

Pechanova Olga (Slovakia) Pokotylo Oleh (Ukraine) Ravingerova Tanya (Slovakia) Singal Pawan K. (Canada) Singh Ram B. (India) Tarnava Alex (Canada)

Supporters

Slovenský plynárenský priemysel, a.s. (SPP) EPH Fundation Ministry of Education, Science, Research and Sport of the Slovak Republic Drink HRW H2 Global Group Antioxidants (MDPI) Gemerka

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

General Information

Venue and date Congress Centre of SAS – Smolenice Castle, Slovak Republic October 17 th – 20 th , 2022 Registration Monday, October 17 th , 2022, 15:00 – 17:00 Language The official language – English Accomodation On the premises of the Castle Information for presenters Oral presentations – 15 min including discussion Poster presentations – 5 min including discussion

Posters should be mounted after the registration ACCORDING TO THEIR NUMBER IN THE LIST OF POSTERS. The authors should be present during the Moderated Poster session at Tuesday 18 th October. Poster size – 80 x 120 cm (vertical).

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

CONTENT PROGRAM OVERVIEW SCIENTIFIC PROGRAM LIST OF POSTERS ORAL PRESENTATIONS Artamonov Mikhail: INTRAOSSEOUS HYDROGEN THERAPY

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Barancik Miroslav: THE EFFECT OF THERAPIES ON MODULATION OF MATRIX METALOPROTEINASES AT PATHOLOGICAL SITUATIONS Botek Michal: MOLECULAR HYDROGEN APPLICATION IN EXERCISE AND TRAINING – CURRENT KNOWLEDGE AND FUTURE PROSPECTS Cinquin Philippe: COVIDROGEN: EXPLORATION IN TWO CLINICAL TRIALS OF THE CLINICAL INTEREST OF HYDROGEN INHALATION AND OF HYDROGEN-RICHWATER INGESTION FOR COVID-19 AMBULATORY OR HOSPITALIZED PATIENTS Firment Jozef: THE BENEFICIAL EFECT OF MOLECULAR HYDROGEN IN INTENSIVE CARE IS AWAITED Ghizal Fatima: EFFECTS OF MOLECULAR HYDROGEN THERAPY ON CIRCADIAN BLOOD PRESSURE VARIABILITY. NOTABLY AT NIGHT Hancock John T.: MOLECULAR TARGETS OF HYDROGEN IN BIOLOGY: WHAT ARE THEY? He Qianjun: NANOMATERIALS – ENABLED PRECISION HYDROGEN THERAPY Hyspler Radomir: THE ROLE OF MOLECULAR HYDROGEN AS HIGHLY BIOAVAILABLE ANTIOXIDANT Kalocayova Barbora: EFFECT OF MOLECULAR HYDROGEN ON CARDIAC SURGERY-ASSOCIATED ACUTE KIDNEY INJURY Khazaei Majid: HYDROGEN-RICH WATER COULD IMPROVE MUSCLE ATROPHY IN A HINDLIMB IMMOBILIZATION MODEL IN A MICE DURING ATROPHY AND RECOVERY PHASES

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Krajicek Milan: CASE STUDY OF MOLECULAR HYDROGEN AS ANTIPYRETICANDCHRONOTHERAPYWITHMOLECULARHYDROGEN Krejci Jakub: DOSE-RESPONSE OF HYDROGEN RICH WATER ON PERCEIVED MUSCLE SORENESS IS MODERATED BY AGE Kura Branislav: THERAPEUTIC EFFECT OF MOLECULAR HYDROGEN ON THE HEART DURING IST SIMULATED TRANSPLANTATION LeBaron Tyler W.: ORP SHOULD NOT BE USED TO ESTIMATE OR COMPARE CONCENTRATIONS OF AQUEOUS H2: AN IN-SILICO AND EXPERIMENTAL ANALYSIS Lee Kyu-Jae: HYDROGEN, LIFE ENERGY Liu Jiankang: HYDROGEN MEDICINE: A RISING STAR IN GAS MEDICINE Long Jiangang: MOLECULAR HYDROGEN – A NOVEL MITOCHONDRIAL NUTRIENT Luby Stefan: HYDROGEN VS. GRAPHENE: SIMILARITIES AND PITFALLS IN THE APPLICATIONS OF TWO PROGRESSIVE MATERIALS Lucas Kurt: DEVELOPMENT OF AN EFFERVESCENT HYDROGEN GENERATING TABLET Ma Xuemei: FROM ENERGY TO EVOLUTION: HYDROGEN METABOLISM IN EUKARYOTES Manal Mohammed Ahmed: EFFECT OF MOLECULAR HYDROGEN THERAPY ON COVID-19 INDUCED CIRCADIAN INCREASE IN BLOOD PRESSURE WITH LOSS OF NIGHT DIPPING Matolin Vladimir: HYDROGEN INHALERS: PRINCIPLE, DESIGN AND SAFETY FEATURES Medvedev Oleg: PERSONALIZED APPROACH TO THE USE OF EXTERNAL MOLECULAR HYDROGEN IN MEDICINE Nazarov Eugeny: EXPERIENCE IN THE USE OF MOLECULAR HYDROGEN AND A MIXTURE OF HYDROGEN-NITRIC OXIDE IN HEART SURGERY UNDER CONDITIONS OF ARTIFICIAL BLOOD CIRCULATION Noda Mami: MODULATION OF ENDOCRINE SYSTEM BY HYDROGEN GAS

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Ohta Shigeo: DEVELOMPENT OF HYDROGEN MEDICINE AND ITS MISSION FOR THE FUTURE Pechanova Olga: NITRIC OXIDE: FROM THE BENEFICAL EFFECTS OF THE SOLO MOLECULE TO COMBINATION THERAPY WITH MOLECULAR HYDROGEN PetrovaMarkantova Zornitza: JURISTICANDOTHER PITFALLS INCLINICAL APPLICATION OF MOLECULAR HYDROGEN IN EUROPE Pokotylo Oleh: RELATIONSHIP BETWEEN MOLECULAR HYDROGEN CONTENT, ORP, PH AND WATER MINERALIZATION IN THERMOSES IONIZERS-GENERATORS “H2 LIVING WATER“ Qin Shucun: ROLE OF HYDROGEN IN ATHEROSCLEROTIC DISEASE: FROM BENCH TO BEDSIDE Ravingerova Tanya: MOLECULAR HYDROGEN POTENTIATES BENEFICIAL EFFECTS OF HYPOXIC POSTCONDITIONING AGAINST ISCHEMIA/ REPERFUSION INJURY IN ISOLATED RAT HEARTS: A NOVEL CARDIOPROTECTIVE INTERVENTION Shen Wenbiao: HYDROGEN AGRICULTURE: FROM FARM TO FORK Shogenova Lyudmila: HYDROGEN INHALATION IN REHABILITATION PROGRAM OF THE MEDICAL STAFF, RECOVED AFTER COVID-19 INFECTION Singal Pawan K.: VITAMIN C MITIGATES DOXORUBICIN-INDUCED NITROSATIVE STRESS Singh Ram B.: CAN MOLECULAR HYDROGEN THERAPY ENHANCE OXYGEN SATURATION AMONG PATIENTS WITH CHRONIC LUNG DISEASE? Sladeckova Barbora: THE EFFECT OF HYDROGEN-RICH WATER ON PERFORMANCE FOLLOWING HIGH INTENSITY INTERVAL TRAINING Slezak Jan: HEART TRANSPLANTATION INNOVATIVE METHOD MITIGATINGOXIDATIVESTRESSBYPERIOPERATIVEADMINISTRATION OF MOLECULAR HYDROGEN Song Guohua: HYDROGEN REDUCES NEUTROPHIL EXTRACELLULAR TRAPS (NETS) FORMATION IN ATHEROSCLEROSIS

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Sumbalova Zuzana: EFFECTS OF MOLECULAR HYDROGEN ON ANTIOXIDANT CONTENT AND MITOCHONDRIA FUNCTION IN PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE Sun Xuejun: HYDROGEN MEDICINE: RESEARCH ADVANCE, CONTROVERSY AND CHALLENGES Tarnava Alex: SOLVING THE DISCREPANCY BETWEEN TRANSLATIONAL AND CLINICAL RESEARCH DOSAGES WITH HIGH-CONCENTRATION HYDROGEN TABLETS Valachova Katarina: MOLECULAR HYDROGEN: THE BENEFICIAL EFFECTS INSCAVENGINGHYDROXIL, ALKYLOXYANDALKYLPEROXYRADICALS Xie Keliang: PERSPECTIVE OF MOLECULAR HYDROGEN IN THE TREATMENT OF SEPSIS Zhao Lin: GUT-DERIVED MOLECULAR HYDROGEN: IMPLICATIONS IN METABOLIC SYNDROME POSTER PRESENTATIONS Andelova Katarina: PROTECTION OF IRRADIATED RAT HEART: EFFECT OF HYDROGEN INTAKE Avan Amir: HYDROGEN, A NOVEL THERAPEUTIC MOLECULE, REGULATES OXIDATIVE STRESS AND APOPTOSIS INMUSCLE ATROPHY OF MICE DURING RECOVERY PHASES Cervena Tereza: EFFECT OF MOLECULAR HYDROGEN ON BIOLOGICAL PROCESSES OFMUCILAIRTM3DTISSUE CULTURES EXPOSEDTOTOTAL ENGINE EMISSIONS Fadeev Nikita: VALIDATION METHANE AND HYDROGEN PORTABLE BREATH ANALYZER AIRE2 THROUGH CALIBRATION GASES Grepl Pavel: EFFECTS OF MOLECULAR HYDROGEN INHALATION ON AUTONOMIC NERVOUS SYSTEM Hassanian Seyed Mahdi: HYDROGEN-RICH WATER REDUCES INFLAMMATORY RESPONSES IN MICE DURING ATROPHY Jarmar Adam: EFFECTS OF INHALED MOLECULAR HYDROGEN ON RECOVERYPROCESS AFTERDOWNHILLWALKINGONTHETREADMILL – PRELIMINARY RESULTS Kuropatkina Tatyana: THE DECREASE OF MEAN ARTERIAL PRESSURE IN RATS WITH MONOCROTALINE-INDUCED PULMONARY HYPERTENSION BREATHING A MIXTURE OF AIR CONTAINING 4% HYDROGEN

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Manal Mohammed Ahmed: EFFECT OF MOLECULAR HYDROGEN THERAPY ON COVID-19 INDUCED CIRCADIAN INCREASE IN BLOOD PRESSURE WITH LOSS OF NIGHT DIPPING Nazarov Eugeny: CONDITIONED REFLEX HORMOLYGOS AND THE PLACE OF HYDROGEN IN THE FAMILY OF MEDICAL GASES Pavelkova Patricia: THE EFFECT OF MOLECULAR HYDROGEN ON RADIATIONINDUCED HEART DISEASE Perinova Ilona: WATER AND AIR ENRICHED WITH PURE H2 GENERATED BY PEMWATER ELECTROLYSIS Russell Grace: THE INFLUENCE OF OXY-HYDROGEN ADMINISTRATION ON THE VIABILITY AND PROLIFERATION OF VIRUS-INFECTED B-LYMPHOCYTES: IMPLICATIONS FOR THE TREATMENT OF COVID-19 AND POST-VIRAL SYNDROME Saman Ezgi : NITRIC OXIDE-DEPENDENT PATHWAY IN METABOLIC SYNDROME: POSSIBLE INTERACTIONS WITHMOLECULAR HYDROGEN Szeiffova Bacova Barbara: HYDROGEN INTAKE ATTENUATES OXIDATIVE STRESS AND INFLAMMATION IN IRRADIATED RAT HEARTS Todorovic Nikola: ACUTE EFFECTS OF HYDROGEN RICH-WATER BATH ON MUSCULAR DAMAGE BIOMARKERS AND SORENESS PERCEPTION AFTER HIGH-INTENSITY ECCENTRIC TRAINING Todorovic Nikola: GENDER-SPECIFIC RESPONSES AFTER MOLECULAR HYDROGEN AND CAFFEINE SUPPLEMENTATION ON ALERTNESS AND BRAIN METABOLISM IN SLEEP-DEPRIVED HEALTHY ADULTS Tuyet Truong Thi: HYDROGEN INHALATION IN REHABILITATION PROGRAM OF MEDICAL STAFF RECOVERED FROM COVID-19 INFECTION Valenta Michal: HYDROGEN RICH-WATER SUPPLEMENTATION DOES NOT IMPROVE RUNNING PERFORMANCE AT MAXIMAL AEROBIC SPEED IN NATIONAL LEVEL TRACK AND FIELD RUNNERS Vlkovicova Jana: THERAPEUTIC EFFECT OF MOLECULAR HYDROGEN ON RADIATION-INDUCED OVERPRODUCTION OF FREE RADICALS IN MYOCARDUM REGISTER OF AUTHORS NOTES

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

PROGRAM OVERVIEW MONDAY, October 17 th

15:00 – 17:00 17:00 – 18:30

Arrival to Smolenice, registration, accommodation

Session I.: Plenary Session

18:30

Welcome Party & Dinner (Red saloon & Dining rooms)

TUESDAY, October 18 th

08:00 – 09:00 09:00 – 10:00 10:00 – 10:45 10:45 – 11:05 11:05 – 11:50 12:00 – 13:00 13:00 – 14:00 14:00 – 14:20 14:20 – 15:35 15:35 – 15:55 15:55 – 16:55

BREAKFAST

Opening ceremony

Session II. (Mechanisms of the Biological and Biomedical Effects of Molecular Hydrogen) PART I. Session III. (Mechanisms of the Biological and Biomedical Effects of Molecular Hydrogen) PART II. COFFEE BREAK and PHOTO

LUNCH

Session IV. (Improvement of Energy Metabolism by Molecular Hydrogen)

COFFEE BREAK

Session V. (Molecular Hydrogen as a New Strategy for the Prevention of Cardiovascular diseases) PART I.

COFFEE BREAK

Session VI. (Technical Aspects of the Preparation and Application of Molecular Hydrogen)

17:00 – 18:30

Moderated Poster session. (Cheese & Wine)

19:00

GET TOGETHER DINNER

WEDNESDAY, October 19 th

08:00 – 09:00 09:00 – 11:30 11:30 – 11:45 11:45 – 12:45 12:45 – 13:45 13:45 – 18:00 19:00 – 22:30

BREAKFAST

Session VII. (Online session – video presentations)

COFFEE BREAK

Session VIII. (Molecular Hydrogen Therapy: a Potential for Patients with COVID-19) Free time. Tour to the Trnava city and a visit of the Sessler Brewery. Farewell party. Announcement of the Winners of Young Investigator Poster Competition. Award Giving Ceremony. LUNCH

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

THURSDAY, October 20 th

08:00 – 09:00 09:00 – 09:45

BREAKFAST

Session IX. (Molecular Hydrogen as a New Strategy for the Prevention of Cardiovascular Diseases) PART II. Session X. (Nanotechnology and Technological Progress Towards Molecular Hydrogen Research)

09:45 – 10:45

10:45 – 11:05 10:45 – 12:00 12:00 – 12:30 12:30 – 13:30

COFFEE BREAK

Business meeting of EAH

Closing remarks

LUNCH Departure

14:00

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

SCIENTIFIC PROGRAM MONDAY, October 17 th 15:00 – 17:00

Arrival to Smolenice, registration, accommodation

17:00 – 18:30

Session I: Plenary session (90 min.) Chairs: Jan Slezak (Slovakia), Branislav Kura (Slovakia)

17:30 – 18:00

Shigeo Ohta (Japan) DEVELOPMENT OF HYDROGENMEDICINE AND ITS MISSION FOR THE FUTURE

18:00 – 18:15

Kyu-Jae Lee (Republic of Korea) HYDROGEN, LIFE ENERGY

18:15 – 18:30

John T. Hancock (United Kingdom) MOLECULAR TARGETS OF HYDROGEN IN BIOLOGY: WHAT ARE THEY?

18:30

Welcome Party & Dinner (Red saloon & Dining rooms)

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

TUESDAY, October 18 th 08:00 – 09:00

BREAKFAST

09:00 – 10:00

Opening ceremony & Artistic performance prof. Peter Michalica (violin) with accompaniment (60 min.) Session III: Biomedical effects of molecular hydrogen (part 1) (45 min.) Chairs: Majid Khazaei (Iran), Oleg Medvedev (Russian Federation) Majid Khazaei (Iran) HYDROGEN-RICH WATER COULD IMPROVE MUSCLE ATROPHY IN A HINDLIMB IMMOBILIZATION MODEL IN A MICE DURING ATROPHY AND RECOVERY PHASES Radomir Hyspler (Czechia) THE ROLE OF MOLECULAR HYDDROGEN AS HIGHLY BIOAVAILABLE ANTIOXIDANT Oleg Medvedev (Russian Federation) PERSONALIZED APPROACH TO THE USE OF THE EXTERNAL MOLECULAR HYDROGEN IN MEDICINE

10:00 – 10:45

10:00 – 10:15

10:15 – 10:30

10:30 – 10:45

10:45 – 11:05

COFFEE BREAK & PHOTO

11:05 – 11:50

Session III: Mechanisms of the biological and biomedical effects of molecular hydrogen (part 2) (45 min.) Chairs: Mikhail Artamonov (U.S.A.), Mami Noda (Japan)

11:05 – 11:20

Mikhail Artamonov (U.S.A.) INTRAOSSEOUS HYDROGEN THERAPY

11:20 – 11:35

Katarina Valachova (Slovakia) MOLECULAR HYDROGEN: THE BENEFICIAL EFFECTS IN SCAVENGING HYDROXYL, ALKOXY- AND ALKYLPEROXY- RADICALS Mami Noda (Japan) MODULATION OF ENDOCRINE SYSTEM BY HYDROGEN GAS

11:35 – 11:50

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

12:00 – 13:00

LUNCH

13:00 – 14:00

Session IV: Improvement of energy metabolism by molecular hydrogen (60 min.) Chairs: Zuzana Sumbalova (Slovakia), Michal Botek (Czechia) Michal Botek (Czechia) MOLECULAR HYDROGEN APPLICATION IN EXERCISE AND TRAINING – CURRENT KNOWLEDGE AND FUTURE PROSPECTS Jakub Krejci (Czechia) DOSE-RESPONSEOFHYDROGENRICHWATERONPERCEIVED MUSCLE SORENESS IS MODERATED BY AGE Zuzana Sumbalova (Slovakia) EFFECTS OF MOLECULAR HYDROGEN ON ANTIOXIDANT CONTENT AND MITOCHONDRIA FUNCTION IN PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE Barbora Sladeckova (Czechia) THEEFFECTOFHYDROGEN-RICHWATERONPERFORMANCE FOLLOWING HIGH INTENSITY INTERVAL Session V: Molecular hydrogen as a new strategy for the prevention of cardiovascular diseases (part I) (75 min.) Chairs: Pawan K. Singal (Canada), Euhenij Nazarov (Ukraine) Euhenij Nazarov (Ukraine) EXPERIENCE IN THE USE OF MOLECULAR HYDROGEN AND A MIXTURE OF HYDROGEN-NITRIC OXIDE IN HEART SURGERY UNDER CONDITIONS OF ARTIFICIAL BLOOD CIRCULATION Jan Slezak (Slovakia) HEART TRANSPLANTATION INNOVATIVE METHOD MITIGATING OXIDATIVE STRESS BY PERIOPERATIVE ADMINISTRATION OF MOLECULAR HYDROGEN COFFEE BREAK

13:00 – 13:15

13:15 – 13:30

13:30 – 13:45

13:45 – 14:00

14:00 – 14:20

14:20 – 15:35

14:20 – 14:35

14:35 – 14:50

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

14:50 – 15:05

Branislav Kura (Slovakia) THERAPEUTIC EFFECT OF MOLECULAR HYDROGEN ON THE HEART DURING ITS SIMULATED TRANSPLANTATION Barbora Kalocayova (Slovakia) EFFECT OF MOLECULAR HYDROGEN ON CARDIAC SURGERY-ASSOCIATED ACUTE KIDNEY INJURY Pawan K. Singal (Canada) VITAMIN C MITIGATES DOXORUBICIN-INDUCED NITROSATIVE STRESS Session VI: Technical aspects of the preparation and application of molecular hydrogen (60 min.) Chairs: Tyler W. LeBaron (U.S.A.), Oleg Pokotylo (Ukraine) Tyler W. LeBaron (U.S.A.) ORP SHOULD NOT BE USED TO ESTIMATE OR COMPARE CONCENTRATIONS OF AQUEOUS H2: AN IN-SILICO AND EXPERIMENTAL ANALYSIS. Milan Krajicek (Czechia) CASE STUDY OF MOLECULAR HYDROGEN AS ANTIPYRETIC AND CHRONOTHERAPY WITH MOLECULAR HYDROGEN Oleg Pokotylo (Ukraine) RELATIONSHIPS BETWEEN MOLECULAR HYDROGEN CONTENT, ORP, PH AND WATER MINERALIZATION IN THERMOSES-IONIZERS-GENERATORS "H2 LIVING WATER" Zornitza Petrova Markantova (Czechia) JURISTIC AND OTHER PITFALLS IN CLINICAL APPLICATION OF MOLECULAR HYDROGEN IN EUROPE Moderated poster session (Cheese & Wine) Evaluators: Tyler W. LeBaron (U.S.A.), Michal Botek (Czechia) & Mami Noda (Japan), John T. Hancock (United Kingdom) COFFEE BREAK

15:05 – 15:20

15:20 – 15:35

15:35 – 15:55

15:55 – 16:55

15:55 – 16:10

16:10 – 16:25

16:25 – 16:40

16:40 – 16:55

17:00 – 18:30

19:00

GET TOGETHER DINNER

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

WEDNESDAY, October 19 th 08:00 – 09:00

BREAKFAST

09:00 – 11:30

Session VII: Online session – video presentations (150 min.) Chairs: Shigeo Ohta (Japan), Kyu-Jae Lee (Republic of Korea) Qianjun He (China) NANOMATERIALS – ENABLED PRECISION HYDROGEN THERAPY

09:00 – 09:15

09:15 – 09:30

Kieliang Xie (China) PERSPECTIVE OF MOLECULAR HYDROGEN IN THE TREATMENT OF SEPSIS

09:30 – 09:45

Lin Zhao (China) GUT-DERIVED MOLECULAR HYDROGEN: IMPLICATIONS IN METABOLIC SYNDROME Jiangang Long (China) MOLECULAR HYDROGEN – A NOVEL MITOCHONDRIAL NUTRIENT Shucun Qin (China) ROLE OF HYDROGEN IN ATHEROSCLEROTIC DISEASE: FROM BENCH TO BEDSIDE Jiankang Liu (China) HYDROGEN MEDICINE: A STAR IN GAS MEDICINE Guohua Song (China) HYDROGEN REDUCES NEUTROPHIL EXTRACELLULAR TRAPS (NETs) FORMATION IN ATHEROSCLEROSIS Wenbiao Shen (China) HYDROGEN AGRICULTURE: FROM FARM TO FORK

09:45 – 10:00

10:00 – 10:15

10:15 – 10:30

10:30 – 10:45

10:45 – 11:00

11:00 – 11:15

Xuejun Sun (China) HYDROGEN MEDICINE: RESEARCH ADVANCE, CONTROVERSY AND CHALLENGES

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

11:15 – 11:30

Ghizal Fatima (India) EFFECTS OF HYDROGEN RICH WATER ON CIRCADIAN VARIATIONS OF BLOOD PRESSURES

11:30 – 11:45

COFFEE BREAK

11:45 – 12:45

Session VIII: Molecular hydrogen therapy: a potential for patients with COVID-19 (60 min.) Chairs: Phillippe Cinquin (France), Ram B. Singh (India) Jozef Firment (Czechia) THE BENEFICIAL EFFECT OF MOLECULAR HYDROGEN IN INTENSIVE CARE IS AWAITED Philippe Cinquin (France) COVIDROGEN: EXPLORATION IN TWO CLINICAL TRIALS OF THE CLINICAL INTEREST OF HYDROGEN INHALATION AND OF HYDROGEN-RICH WATER INGESTION FOR COVID-19 AMBULATORY OR HOSPITALIZED PATIENTS Lyudmila Vladimirovna Shogenova (Russian Federation) HYDROGEN INHALATION IN REHABILITATION PROGRM OF THE MEDICAL STAFF, RECOVERED AFTER COVID-19 INFECTION Ram B. Singh (India) CANMOLECULARHYDROGENTHERAPY ENHANCE OXYGEN SATURATION AMONG PATIENTS WITH CHRONIC LUNG DISEASE?

11:45 – 12:00

12:00 – 12:15

12:15 – 12:30

12:30 – 12:45

12:45 – 13:45

LUNCH

13:45 – 18:00

Free time. Tour to the Trnava city and a visit of the Sessler Brewery.

19:00 – 22:30

Farewell party. Announcement of the Winners of Young Investigator Poster Competition. Award Giving Ceremony.

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

THURSDAY, October 20 th 08:00 – 09:00

BREAKFAST

09:00 – 09:45

Session IX: Molecular Hydrogen as a new strategy for the prevention of cardiovascular diseases (part 2) (45min) Chairs: Tanya Ravingerova (Slovakia), Lyudmila Vladimirovna Shogenova (Russia) Miroslav Barancik (Slovakia) THE EFFECT OF THERAPIES ON MODULATION OF MATRIX METALLOPROTEINASES AT PATHOLOGICAL SITUATIONS Olga Pechanova (Slovakia) NITRIC OXIDE: FROM THE BENEFICIAL EFFECTS OF THE SOLO MOLECULE TO COMBINATION THERAPY WITH MOLECULAR HYDROGEN Tanya Ravingerova (Slovakia) MOLECULAR HYDROGEN POTENTIATES BENEFICIAL EFFECTS OF HYPOXIC POSTCONDITIONING AGAINST ISCHEMIA/REPERFUSION INJURY IN ISOLATED RAT HEARTS: A NOVEL CARDIOPROTECTIVE INTERVENTION Session X: Nanotechnology and technological progress towards molecular hydrogen research (60 min.) Chairs: Vladimir Matolin (Czechia), Kurt Lucas (Germany) Stefan Luby (Slovakia) HYDROGEN VS. GRAPHENE: SIMILARITIES AND PITFALLS IN THE APPLICATIONS OF TWO PROGRESSIVE MATERIALS Vladimir Matolin (Czechia) HYDROGEN INHALERS: PRINCIPLE, DESIGN AND SAFETY FEATURES Alex Tarnava (Canada) SOLVING THE DISCREPANCY BETWEEN TRANSLATIONAL AND CLINICAL RESEARCH DOSAGES WITH HIGH – CONCENTRATION HYDROGEN TABLETS

09:00 – 09:15

09:15 – 09:30

09:30 – 09:45

09:45 – 10:45

09:45 – 10:00

10:00 – 10:15

10:15 – 10:30

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

10:30 – 10:45

Kurt Lucas (Germany) DEVELOPMENT OF AN EFFERVESCENT HYDDROGEN – GENERATING TABLET

10:45 – 11:05

COFFEE BREAK

10:45 – 12:00

BUSINESS MEETING OF EAH

12:00 – 12:30

CLOSING REMARKS

12:30 – 13:30

LUNCH

14:00

DEPARTURE

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

LIST OF POSTERS 1. ♣ ANDELOVA, SZEIFFOVA BACOVA, SYKORA, KALOCAYOVA, ZALESAK, TRIBULOVA, SLEZAK, KURA. Protection of irradiated rat heart: effect of hydrogen intake (Bratislava, Slovakia) 2. ♣ AVAN, BABAIE, FERNS. Hydrogen, a novel therapeutic molecule, regulates oxidative stress and apoptosis in muscle atrophy of mice during recovery phases (Mashad, Iran) 3. ♣ CERVENA, TOPINKA, MARGARYAN, VIMROVA, VOJTISEK-LOM, ROSSNER JR. Effect of molecular hydrogen on biological processes of mucilairtm 3d tissue cultures exposed to total engine emissions (Prague, Czechia) 4. ♣ FADEEV, KRIVETSKY, MEDVEDEV. Validation methane and hydrogen portable breath analyzer aire2 through calibration gases (Moscow, Russian Federation) 5. ♣ GREPL, BOTEK, KREJCI. Effects of molecular hydrogen inhalation on autonomic nervous system (Olomouc, Czechia) 6. ♣ HASSANIAN, ASGHARZADEH, KHALILI, RYZHIKOV. Hydrogen-rich water reduces inflammatory responses in mice during atrophy (Mashad, Iran) 7. ♣ JARMAR, BOTEK, VALENTA, SLADECKOVA, NEULS, VYHNANEK, SADIL. Effect of inhaled molecular hydrogen on recovery process after downhill walking on the treadmill – preliminary results (Olomouc, Czechia) 8. ♣ KUROPATKINA, SYCHOV, ARTEMYEVA, SHYROKOV, SAMOYLENKO, GERASIMOVA, SHISHKINA, MEDVEDEVA, MEDVEDEV. The decrease of mean arterial pressure in rats with monocrotaline-induced pulmonary hypertension breathing a mixture of air containing 4% hydrogen (Moscow, Russian Federation) 9. ♣ MANAL, SHARMA, SINGH. Effect of molecular hydrogen therapy on Covid-19 induced circadian increase in blood pressure with loss of night dipping (Abu Dhabi, United Arab Emirates) 10. NAZAROV, NODA. Conditioned reflex hormolygos and the place of hydrogen in the family of medical gases (Odessa, Ukraine) 11. PAVELKOVA, KALOCAYOVA, SLEZAK, KURA. The effect of molecular hydrogen on radiation-induced heart disease (Bratislava, Slovakia) 12. ♣ PERINOVA, HORACKOVA. Water and air enriched with pure H2 generated by pem water electrolysis (Ceske Mezirici, Czechia) 13. ♣ RUSSELL, THOMAS, NENOV, HANCOCK. The influence of oxy-hydrogen administration on the viability and proliferation of virus-infected b-lymphocytes: implications for the treatment of covid-19 and post-viral syndrome (Bristol, UK)

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

14. ♣ SAMAN, PECHANOVA. Nitric oxide-dependent pathway in metabolic syndrome: possible interactions with molecular hydrogen (Bratislava, Slovakia) 15. SZEIFFOVA BACOVA, SYKORA, MARTISKOVA, TRIBULOVA, ANDELOVA, KALOCAYOVA, SLEZAK, KURA. Hydrogen intake attenuates oxidative stress and inflammation in irradiated rat hearts (Bratislava, Slovakia) 16. ♣ TODOROVIC, JAVORAC, STAJER, OSTOJIC. Acute effects of hydrogen rich water bath on muscular damage biomarkers and soreness perception after high-intensity eccentric training (Novi Sad, Serbia) 17. ♣ TODOROVIC, ZANINI, STAJER, KOROVLJEV, OSTOJIC, OSTOJIC. Gender specific responses after molecular hydrogen and caffeine supplementation on alertness and brain metabolism in sleep-deprived healthy adults (Novi Sad, Serbia) 18. ♣ TRUONG, SHOGENOVA, KRYUKOVA, YUSUPKHOJAEVA, POZDNYAKOVA, KIM, CHERNYAK, KALMANOVA, MEDVEDEV, KUROPATKINA, VARFOLOMEEV, RYABOKON, SVITICH, KOSTINOV, KUNIO IBARAKI, HIROKI MAEHARA, CHUCHALIN. Hydrogen inhalation in rehabilitation program of medical staff recovered from COVID-19 infection (Moscow, Russian Federation) 19. ♣ VALENTA, BOTEK, KREJČÍ, MCKUNE, SLÁDEČKOVÁ, KLIMEŠOVÁ, NEULS. Hydrogen rich water supplementation does not improve running performance at maximal aerobic speed in national level track and field runners (Olomouc, Czechia) 20. VLKOVICOVA, KURA, KALOCAYOVA, KORNIEIEVA, SYKORA, KLUKNAVSKY, CACANYIOVA, ZORAD, POBIJAKOVA, SAGATOVA, FULOP, PAVELKOVA, FERENCZYOVA, SLEZAK. Therapeutic effect of molecular hydrogen on radiation induced overproduction of free radicals in myocardium (Bratislava, Slovakia)

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

ORAL PRESENTATIONS

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Artamonov Mikhail

Dr. Mikhail Artamonov referred affectionately and syllable-saving as “Dr A” has a complex and woven background but is guided by one simple credo: the practice of Good Medicine. He believes that new medical technologies not only make medicine more effective but can actually make it more personal. He is the visionary and inspiration behind MJA HealthCare Network. Born in Moscow, Russia, his earliest experience was as an Ob-Gyn Nursing Aide at the age of 14. Dr. Artamonov has a unique background and is the only physician in the US who is board certified in 8 different areas of medicine.

Trained all over the world, he has been applying different ozone technique in his practice for over 20 years. Originally trained in Russian and German schools of Ozonotherapy, he was a founding member of American Academy of Ozonotherapy and one of the first three doctors receiving certification in this field in the US. Besides basic applications, he is specializing in intradiscal and EBOO therapy. He is developer of 99 Body Purify Total Body Dialysis™, Spinal Dialysis™ and many other novel treatments. Dr. A graduated from Moscow Medical Sechenov Academy in 1994 with MD degree and the same year with MA degree from Moscow University Oriental Languages Department specializing in Chinese studies. Subsequently, he studied different schools of acupuncture and herbology in China, South Korea and Japan. He graduated fromthefirst inworld residencyprogramin IntegrativeMedicine, studyingdifferentmethods of natural healing, ethnic medicine and many others. He finished Neurology and Psychiatry residency program in Russia and Germany. Studied homeopathy, ozonotherapy and psychotherapy in Germany. He defended his PhD thesis in clinical medicine in the field of prolotherapy and knee osteoarthritis treatment. Over 30 years of his non-stop search around our planet for the best medical knowledge he became trained in different specialties including Neurology and Physiatry, Anesthesiology and Psychiatry, Internal and Integrative Medicine. He brought together unique technologies from all over world to achieve the best for his patients. In 1999, He entered his carrier path in the US, finishing Internal Medicine Internship in famous Brooklyn Jewish hospital and Physical Medicine and Rehabilitation residency at Temple University in 2003. Subsequently, he was awarded the only one annual position in the combined Harvard Medical School Fellowship in Interventional Pain Medicine in both adults and children, working in all major teaching hospitals. He also finished Fellowship in Functional medicine from A4M, attended many post-graduate courses and specialties, constantly looking for new and better medical technologies to help his patients from all over the world. Dr A is the Founder and President of World Congress of Intraosseous Therapy, international non profit society, representing the international medical and scientific community interested in further development of this exciting method of treatment. He is teaching basic and advanced workshops and master classes on different techniques. He is creator and developer of several unique applications of IOT including Spinal Dialysis™, Intraosseous Photodynamic Therapy™, End-plate™ method etc. He is the author of several books and is regularly publishing in peered review journals on different topics. He is also running successful R&D company developing new methods, protocols and intel property in many areas of medicine and biology.

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

INTRAOSSEOUS HYDROGEN THERAPY This is the first in the world presentation on the topic of Intraosseous Hydrogen Therapy taught by the President of World Congress of Intraosseous Therapy and American Society of Medical Gases, Dr. Mikhail Artamonov, MD PhD. Scientific basis and theories behind the Interosseous Therapy, different clinical protocols and scenarios will be presented. The audience should be able to start applying this unique method in the practice after specialized training. The Intraosseous Therapy (OIT) method including injections and infusions method has been successful in the treatment of different pain syndromes and beyond. Intraosseous blockade is a highly effective way to treat pain and associated sensory, motor, spastic, vascular and other disorders by injecting a drug solution into the bone tissue. The application of IOT is based on the concept of osteogenic etiology, explaining the mechanisms of pain syndromes in many neurological and orthopedic diseases. Thus, by using IOT it is possible to reduce the intraosseous pressure and bone marrow edema, which in turn should lead to a regression in the clinical symptoms and morphological changes. IOT helps heal and nourish the degenerative cartilage from the bone and joint inside out. It is more advanced non-surgical regenerative option, and a more natural way to nourish and heal degenerated joints. Molecular hydrogen was reported as a preventive and therapeutic antioxidant that selectively scavenges hydroxyl radical and peroxynitrite and improves bone marrow damage. Possible effects of Intraosseous Hydrogen on bone marrow delivered by IOT method are reviewed. Basic IOT techniques does not require navigation equipment and the risk of complications and side effects is minimized. No sedation is required, can be done in office setting - your patients will be able to return home the same day after receiving these injections. More advanced IOT techniques, additional indications and protocols for wide variety of diseases including Parkinson, Alzheimer’s’, post-stroke recovery and other will be discussed. One of his favorite areas onmedicine has seenmedical gases. He is Founder and President of American Society of Medical Gases, has been on advisory and scientific boards of different international associations. He is always staying on the top of major developments in the field of medicine, biology and biophysics bringing the best treatments for his patients.

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Barancik Miroslav

Is a Senior Scientist at Centre of Experimental Medicine of Slovak Academy of Sciences (CEM SAS). Current position: Director of Institute for Heart Research CEM SAS. His scientific activities are focused on intracellular and redox signaling and study of molecular mechanisms underlying the responses of animal cells to pathological conditions. During his scientific career, he has published more than100 articles in peer-reviewed scientific journals and these publications were cited more than 1,500-times.

THE EFFECT OF THERAPIES ON MODULATION OF MATRIX METALOPROTEINASES AT PATHOLOGICAL SITUATIONS Institute for Heart Research, Center of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia BACKGROUND: Matrix metalloproteinases (MMPs) are enzymes that play an important role in degradation and remodeling of extracellular matrix. These proteins are also suggested to play an important role in pathogenesis of several diseases. OBJECTIVES: The current study investigated the potential role of MMPs in pathology of radiation-induced cardiovascular toxicity in rats. Moreover, the effects of molecular hydrogen onMMPs during development of effects induced by mediastinal irradiation (MI) were studied. Determined were also effects of molecular hydrogen on some regulatory proteins (superoxide dismutase, Akt kinase). METHODS: In the study were used male Wistar rats exposed to mediastinal irradiation and/or molecular hydrogen therapy. Samples of heart tissue or plasma were used for further investigations. MMPs activities were determined by gelatin zymography, protein levels of MMPs and specific proteins were determined by Western blot analysis. RESULTS: We found that effects of MI treatment were associated with significant changes of circulating 72 kDa MMP-2 activities. These activities reached maximal levels in plasma collected 2 days after exposure of animals to irradiation. Effects of irradiation on MMP 2 activities were transient and with prolongation of time were reduced. Application of hydrogen during irradiation reduced the negative effects of irradiation on MMP-2 and these effects were associated with modulation of Akt kinase activation and superoxide dismutase protein levels. CONCLUSIONS: Application of molecular hydrogen protected against mediastinal irradiation-induced negative effects on MMP-2. Supported by grants: APVV-18-0548, APVV-19-0317, APVV-15-0376.

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Botek Michal

Is a member of the Department of Natural Sciences in Kinanthropology, Faculty of Physical Culture, Palacky University Olomouc, where he is responsible for teaching Exercise and Sports Physiology. Currently, his primary research focus is on the application of molecular hydrogen supplementation in sports medicine and health sciences, the physiological response to normobaric hypoxia, and autonomic cardiac assessment via heart rate variability. He is a member of the European Academy for Molecular Hydrogen Research in Biomedicine. To date, he has published 41 scientific articles, with 351 citations, and has an h-index of 11.

MOLECULAR HYDROGEN APPLICATION IN EXERCISE AND TRAINING – CURRENT KNOWLEDGE AND FUTURE PROSPECTS Department of Natural Sciences in Kinanthropology, Faculty of Physical Culture, Palacký University Olomouc, Olomouc, Czech Republic BACKGROUND: The unique bio-properties of molecular hydrogen (H2) together with its ergogenic and recovery enhancing effects have resulted in increased research interest over the past two decades. OBJECTIVES: The primary focus of our research has been to investigate the ergogenic effects of H2 across different modes of exercise and populations. We have evaluated the performance and recovery benefits after H2 supplementation, with the results providing initial guidelines for H2 application in training and competition. METHODS: All studies were designed as randomized, double-blinded placebo-controlled cross-over trials. H2 supplementation was performed via hydrogen rich water (HRW) consumption that had the following characteristics, pH = 7.9, oxidation-reduction potential = −652 mV, and dissolved H2 concentration = 0.9. ppm. The supplementation strategy and dose of HRWvaried across studies depending on the specific mode of exercise and exercise protocol design. RESULTS: An ergogenic effect of HRW intake was found for the following modes of exercise. There was an ~2% improvement in running time during the last 30m sprint of 15x30 sprints in professional soccer players. HRW elicited better muscle function during the last 20 repetitions of lunges that were performed 8% faster compared to placebo group. HRW significantly reduced exercise and post-exercise lactate response, and alleviated delayed onset of muscle soreness within 24 h of recovery after a strength training session. In addition, the magnitude of the ergogenic effect of HRW on up-hill running performance depended on individual athlete performance level, with a greater ergogenic effect occuring in lower level athletes. CONCLUSION: Acute, pre-exercise HRW supplementation seems to be an effective strategy for performance enhancement. However, there are further issues that need to be solved. For instance, the effect of chronic H2 application on the adaptation and recovery response in functional overreached/overtrained athletes.

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Cinquin Philippe

Mathematics and Medical Doctor, is Professor of Digital Public Health in Grenoble at Univ. Grenoble Alps (UGA) and Grenoble University Hospital (CHUGA). He is the former head of TIMC (UGA & CNRS), and belongs to Centre for Clinical Investigation (INSERM& CHUGA). He pioneered Computer-Assisted Medical Interventions. He is co-author of 146 papers and 43 patents. He focused on the design of implantablemedical devices capable to scavenge energy, and recently on therapeutic applications of hydrogen. He received theMuller Award for excellence in computer-assisted orthopedic surgery, the CNRS Silver and Innovation Awards, and was finalist for the European Inventor Award for a Glucose BioFuel Cell capable to power implanted medical devices. He holds “Deep Care” (a research chair of Grenoble Multidisciplinary Institute of Artificial Intelligence). COVIDROGEN: EXPLORATION IN TWO CLINICAL TRIALS OF THE CLINICAL INTEREST OF HYDROGEN INHALATION AND OF HYDROGEN-RICHWATER INGESTION FOR COVID-19 AMBULATORY OR HOSPITALIZED PATIENTS J. P. Alcaraz 1 , M. Barbado 2 , J. L. Bosson 1 , 2 , F. Boucher 1 , J. P. Brion 3 , P. Cinquin 1,2, B. Degano 4 , C. Eychenne 1 , J. Giai 1,2, C. Ihl 1 , Y. Gaboreau 1 , A. Lefaivre 1 , M. Margotton 3 , A. Milovančev 5 , A. Moreau-Gaudry 1,2 , S. Ostojic 5 , A. Paris 2 , P. Pavèse 3 , C. Rolland 1 , M. Roustit 2 , S. Silvent 1,2, BACKGROUND: Hydrogen-Therapy was recommended very early by Chinese recommendations for Covid patients. Several biochemical pathways by which molecular hydrogen (H2) could prevent the cytokine storm of COVID have been described. OBJECTIVES: demonstrate that: i) early ingestion of Hydrogen-Rich Water (HRW) in mild ambulatory patients could limit complications of COVID; ii) H2 inhalation can safely be proposed to moderate Covid hospitalized patients. METHODS: HydroCovid is a randomized, triple-blind, multicentric and international clinical trial. Included patients are mild ambulatory patients starting Covid, aged 18 or more, with at least one risk factor. Patients ingested twice a day 250 mL of HRW (with tablets containing 80 mg of reduced Mg, donated by DrinkHRW - Canada) or placebo, for 21 days, immediately after diagnosis. Composite Primary Endpoint includes symptom worsening (dyspnea and fatigue), oxygen use, hospitalization and death occurring within 12 to 14 days of inclusion. Patient follow-up is planned for 12 months, to assess benefit of HRW in long Covid. H2-COVID is a phase 1 clinical trial. Covid patients inhaling S. Tanguy 1 , H. Terrisse 1 , S. Touati 2,3, C. Vermorel 1 1 TIMC (Univ. Grenoble Alpes, CNRS, France), 2 CIC1406 (Grenoble Alpes University Hospital, INSERM, France), 3 Service de Maladies Infectieuses (CHU Grenoble Alpes, France), 4 Service de Pneumologie (CHU Grenoble Alpes, France), 5 Faculty of Medicine (University of Novi Sad, Serbia.

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

< 6L/min by the cannula received a flow of 1 L/min of a medical grade mixture of [3.6% H2; 96.4% N2] prepared by AirProducts (Spain). RESULTS: 700 patients were included for HydroCovid. No statistically significant difference was found on the Primary Endpoint between the intervention and placebo arms (46% of complications versus 44%). 12 patients were included for H2-Covid. Exposure Duration Limiting Toxicity was proven to be at least 6 days. Very encouraging results were observed, with very unusual sharp decrease of inflammatory markers such as CRP or of abnormalities of pulmonary CT. CONCLUSIONS: HRW at the dose of 2 x 250 mL/day did not reduce immediate complications of ambulatory Covid patients. Long-term follow-up is on-going, to explore potential impact on long-Covid. Our protocol of H2 inhalation is safe and can be used for further clinical trials for moderate Covid patients requiring oxygen-therapy, with very encouraging clinical results. ACKNOWLEDGEMENTS: we thank Drink HRW (donation of the Mg tablets + grant), AirProducts (donation of the medical gas + specific gas distribution equipment) and individual donators who supported HydroCovid by donations to Univ. Grenoble Alpes Foundation.

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1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Firment Jozef

Dr. Firment is an associate professor of PJ Šafárik University in Košice. He works as the head of Department of Anaesthesiology and Intensive Care Medicine at the University Hospital. He is currently a member of a research team interested in the implementation of advanced pulmonary ventilation methods for the treatment of severe pneumonia. His publications are on the diagnosis and treatment of sepsis and septic shock and on the monitoring of critically ill patients. THE BENEFICIAL EFECT OF MOLECULAR HYDROGEN IN INTENSIVE CARE IS AWAITED J. Firment, J. Capková, J. Simonova, V. Hudak, M. Grochova Department of Anaesthesiology & Intensive Care Medicine, L. Pasteur University Hospital, Faculty of Medicine PJ Šafárik University in Košice, Slovak Republic CONCLUSIONS: Hydrogen therapy is shown to be effective and promising in the treatment of multiple organ failure syndrome (MODS). Clinical and experimental studies suggest that hydrogen therapy may provide benefits in the treatment of postresuscitation syndrome and other ischemia-reperfusion injuries, stage IV colorectal cancer, stroke, dyslipidaemia, endothelial dysfunction, atherosclerosis. Treatment withH2 can also bemediated by probiotics. At the authors' workplace, there is anH2 generator AMS-H-03 formedical use, which produces by electrolysis H2 66% : O2 33% with a production of 2-3 l H2/min. The gaseous form of H2 can be applied through a nebulizer with a nasal catheter or connected to the inspiratory branch of the ventilator. The created HRW can be used to produce sterile HRS in a plastic bag. Questions to be answered: a) How to avoid the risk of explosion? b) How to determine the administration technique taking into account H2 leakage from the bottles and through the walls of the hoses? c) To verify the effectiveness of 2% concentration of H2 in the inspiratory mixture. d) To determine the dosage of H2 in specific indications for critically ill patients. e) What are the expected adverse effects of hydrogen administration? Are we endangering the natural inflammatory processes of the tissue's defence reaction? The pleiotropic effects of molecular hydrogen on various proteins, molecules, and signalling pathways may at least partially explain its near-universal pluripotent therapeutic potential. Intensive negotiations are currently underway aimed at registering the use of molecular hydrogen for medical purposes in the Czech Republic, and we expect a change in legislation in the Slovak Republic as well. This publication is the result of the project implementation: „Design and implementation of advanced methods of lungs ventilation treatment and diagnosis of viral pneumonia, including Covid-19 with the possibility of their rapid adoption“, ITMS2014+: 313011ASX1 supported by the Operational Programme Integrated Infrastructure, funded by the ERDF.

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October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Ghizal Fatima EFFECTS OF MOLECULAR HYDROGEN THERAPY ON CIRCADIAN BLOOD PRESSURE VARIABILITY. NOTABLY AT NIGHT Ram B Singh, Ghizal Fatima, Aminat Magamedova, JP Sharma, Divyank Sharma 1 Halberg Hospital and Research Institute, Moradabad, India, Era Medical College, Ghizal Fatima, 4 Lomosonov Moscow State University, Moscow, Russia. BACKGROUND: The earth revolves around its axis, causing the light and dark cycles of 24-Hours. resulting in to day and night. These rotations of the planet are associated with alteration in physiological functions which vary according to time structure of 24 hours. OBJECTIVES: This study aims to emphasize that molecular hydrogen therapy in patients with circadian increase of blood pressure (BP) may reverse Covid-19 induced loss of dipping. METHODS ANDRESULTS: Adverse effects of high blood pressure (BP) on the heart correlate better with 24-hour means of BP than with casual BP readings, to which nocturnal BP, and morning rise inBP, plays aparticularly important role [8-10].Under usual conditions, nighttime BP is typically lower than daytime BP. Elevated nocturnal BP (nocturnal hypertension) and disrupted circadian variation in BP emerged as independent risk factors for cardiovascular disease (CVD) and all-cause mortality. Variability in BP and HR stem from an endogenous circadian rhythm, which persists under constant conditions, and from a host of external factors, including an influence from space weather and climate. Determinants of the circadian rhythm in BP are still poorly understood. It is not well known whether they are similar or different during the day or night and how that may interact with sleep and night shift work. Night shift work is known to cause sleep disturbance, increase in night time BP along with oxidative stress and inflammation due to increase in cortisol and decrease in melatonin. While hypertensive patients with COVID-19 appear to have a poor prognosis, there is no evidence that hypertension increases the risk of new infection or adverse outcomes independent of age and other risk factors. It has been suggested, however, that hypertension and BP variability are exacerbated by acute SARS-CoV-2 immune reactions, that has been observed in a male physician, 77 years of age, presenting with COVID-19 in 2021. He had severe insomnia and increase in night-time blood pressure (BP) as shown by self-recorded data. Supplementation with hydrogen-rich water (HRW) increased oxygen saturation 10- 45 minutes after its administration in a patient with COVID-19-like symptoms. We also found that treatment with HRW in other patients with night shift work (n=9) with nocturnal rise in BP (BP >110/75 mm Hg) was beneficial in improving nocturnal increase in BP (loss of dipping) in majority of the patients. There was a significant increase in melatonin and a decrease in cortisol among these patients. After a week, BP measurements showed that BP was reduced, notably at night. Systolic(S) and diastolic(D) BP, which averaged 127.2/84.9 mmHg in April, decreased to 113.5/79.5 mmHg in June (P<0.001). It doubled in the case of SBP and DBP and increased by 85% in the case of heart rate (HR). The difference between daytime (09:00 and 13:00) and night-time (02:30 and 05:30) measurements also increased between April and June: from 1.8

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