Program and book of abstracts 1st conference

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Ravingerova Tanya

Dr. Tanya Ravingerova, MD, PhD, DSc, FIACS is a Senior Scientist at the Department of Cardiovascular Physiology and Pathophysiology, Institute for Heart Research CEM SAS in Bratislava, Slovakia. Visiting professorship: Norway, Canada, UK, Austria. Her research interests are heart ischemia/reperfusion, arrhythmias and sudden death, myocardial protection against ischemic injury in normal and diseased heart; diabetic heart, molecular mechanisms of adaptation and endogenous cardioprotection, conditioning protection, protective cell signaling. She published >140 papers, H-index 33. Orcid : https://orcid.org/0000-0002 8804-6964. Other professional activities: Elected Fellow of the International Academy of Cardiovascular Sciences (FIACS); IACS Executive Council Member; Vice-President of the IACS European Section, Council member of the Czech –Slovak Working group on Experimental cardiology. Awards and distinctions: Medal of the Slovak Cardiological Society & Slovak Medical Association, Medal of the Slovak Physiological Society, Jan Jessenius Medal of the Slovak Academy of Sciences, IACS Distinguished Leadership Award in Cardiovascular Sciences. MOLECULAR HYDROGEN POTENTIATES BENEFICIAL EFFECTS OF HYPOXIC POSTCONDITIONING AGAINST ISCHEMIA/REPERFUSION INJURY IN ISOLATED RAT HEARTS: A NOVEL CARDIOPROTECTIVE INTERVENTION T. Ravingerova 1 , M. Zalesak 1 , B. Kura 1 , J. Graban 2 , V. Farkasová 1 , J. Slezak 1 1 Institute for Heart Research, Center of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia 2 Institute of Experimental Endocrinology, Biomedical Centre, Slovak Academy of Sciences, Bratislava, Slovakia BACKGROUND: Ischemic preconditioning (IPC) and post-conditioning (postC) induced by short-term episodes of ischemia or hypoxia (HpostC) have been shown to enhance heart resistance against sustained long-term ischemia/reperfusion (I/R) injury associated with massive generation of free radicals. Antioxidant therapy attenuates its damaging effects but may also abrogate cell signaling and redox metabolic reactions. However, diatomic hydrogen (H2) acts as a therapeutic and preventive antioxidant able to reduce detrimental hydroxyl radicals (•OH) and peroxynitrite (ONOO-) production in cells and preserves metabolic processes, as well as cell signaling mediated by nitric oxide radical (NO) and superoxide anion (•O2-). OBJECTIVES: This study aimed to explore cardioprotective potential of HpostC against I/R (30-min global I/120-minR) injury in isolated rat hearts usingO2-freeKrebs-Henseleit buffer (KHB) and to evaluate the potential of H2 to facilitate the effect of HpostC. METHODS. HPostC was induced by 4 cycles of 1-min perfusion with O2-free KHB intercepted by 1-min perfusion with normal KHB, at the onset of reperfusion. H2+HPostC was applied in a similar manner using H2-enriched O2-free KHB. Cardioprotective effects were evaluated on the basis of infarct size (IS, in % of area at risk, AR) reduction, post-I/R recovery of heart function and occurrence of reperfusion arrhythmias.

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