Program and book of abstracts 1st conference

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Vlkovicova Jana

Dr Jana Vlkovičová, PhD is an independent scientist at the Centre of Experimental Medicine (CEM) of the Slovak Academy of Sciences (SAS). She is interested in the researchof the properties of Na,K-ATPase, one of the key systems formaintaining the concentration of sodium in the body, in conditions of civilization diseases such as hypertension, diabetes mellitus, and hypertriglyceridemia. She measures the activity of Na, K-ATPase and determines its kinetic parameters (Km, KNa, and Vmax). She uses various methods focused mainly on monitoring enzymatic activity and protein expression. She is also interested in the question of the possible influence of H2 on biochemical parameters. The results of her scientific work were

published in more than 20 scientific papers published in domestic and foreign impacted CC journals. THERAPEUTIC EFFECT OF MOLECULAR HYDROGEN ON RADIATION-INDUCED OVERPRODUCTION OF FREE RADICALS IN MYOCARDUM Jana Vlkovicova 1 , Branislav Kura 1 , Barbora Kalocayova 1 , Daria Kornieieva 1 , Matus Sykora 1 , Michal Kluknavsky 2 , Sona Cacanyiova 2 , Stefan Zorad 3 , Margita Pobijakova 4 , Andrea Sagatova 5 , Marko Fulop 5 , Patricia Pavelkova 1 , Kristina Ferenczyová 1 , Jan Slezak 1 1 Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia 2 Centre of Experimental Medicine, Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovakia 3 Institute of Experimental Endocrinology, Biomedical Research Center, Department of Endocrine Regulations and Psychofarmacology, Slovak Academy of Sciences, Bratislava, Slovakia 4 Department of Radiation Oncology, National Cancer Institute, Bratislava, Slovakia 5 Slovak Medical University, Bratislava, Slovakia BACKGROUND: As the smallest antioxidant, molecular hydrogen (H2) has proven beneficial in numerous animal models and human cardiovascular diseases since 2007. Unlike any other antioxidant, this molecule quickly diffuses into the cells and acts as an effective scavenger of reactive oxygen species (ROS). Moreover, as a potential therapeutic agent, H2 can scavenge hydroxyl radicals generated by ionizing radiation (IR) to reduce free radical production during radiotherapy. Modifying endogenous antioxidant activity is crucial to preventing oxidative stress-related pathologies. OBJECTIVES: By comparing the effectiveness of two alternative methods of H2 delivery - hydrogen-rich water (HRW) administration and H2 inhalation - this study examined the effects of a potential therapeutic medical gas on laboratory animals undergoing mediastinal IR. Three-month-old male rats were irradiated with a single dose of 10 Gy and treated with HRW (2 mg/L) or inhalation (30 min of 4% H2 gas) daily three times for two and nine days, respectively. RESULTS: HRW administration and the inhalation of H2 significantly improved blood biochemical parameters (lactate dehydrogenase, alanine aminotransferase, uric acid, etc.), where the inhalation was more effective. Further, oxidative stress and inflammation markers (superoxide dismutase, catalase, malondialdehyde, TNF-α and NF-κB) were significantly

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