Program and book of abstracts 1st conference

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Kuropatkina Tatyana

Is a PhD student at Lomonosov Moscow State University, working as a laboratory research assistant. Research interests include investigations of antioxidants on different animal models of Pulmonary Hypertension. The objectives are to find an effective adjuvant therapy to support people suffering from diseases connected with oxidative stress and hardly pharmacology corrected like Pulmonary Hypertension. Has several publications in Russian and foreign periodicals about the influence of CoQ10 and H2. THE DECREASE OF MEAN ARTERIAL PRESSURE IN RATS WITH MONOCROTALINE-INDUCED PULMONARY HYPERTENSION BREATHING A MIXTURE OF AIR CONTAINING 4% HYDROGEN T. Kuropatkina 1 , F. Sychov 1 , M. Artemyeva 1 , I. Shyrokov 2 , T. Samoylenko 3 , O. Gerasimova 3 , V. Shishkina 3 , N. Medvedeva 4 , O. Medvedev 1 1 Faculty of fundamental medicine, Lomonosov Moscow State University, Moscow, Russia, 2 Department of Biological andMedical Physics, BaumanMoscow State Technical University, Moscow, Russia, 3 Research Institute of Experimental Biology and Medicine, Voronezh, Russia, 4 Biological faculty, Lomonosov Moscow State University, Moscow Russia. BACKGROUND: The pathogenesis of monocrotaline-induced pulmonary hypertension (MCT-PH) is associated with oxidative stress and inflammation. OBJECTIVES: The aim of the work was to investigate the influence of 4% hydrogen in the air on biomarkers of 3-week MCT-PH development in male rats. METHODS: MCT-PH was induced by single s/c injection of MCT alcohol solution (60 mg/kg, Sigma Aldrich) to male Wistar rats (MCT-group, n=16). Control animals were injected with vehicle only (Control group, n=8). The MCT-group was divided into two groups: MCT-Control rats (n=8) were kept in boxes ventilated with room air, and MCT-H2 rats, which were ventilated with air containing 4% hydrogen for three weeks. After 21 days, right ventricular systolic pressure (RVSP) and mean systemic pressure (MSP) were measured in all animals under urethane anesthesia. In addition, right ventricular hypertrophy (RVH) was assessed andhistological analysis of lung tissuewas performed, inwhich the number and quality ofmast cells (MCs) were evaluated. RESULTS: There was a significant increase in RVSP in MCT-Control (57±9 mm Hg) and MCT-H2 (58±9mmHg) groups in comparison to the Control-group (37±5mmHg) (p<0.01), indicating the development of MCT-PH. No differences were found between the MCT groups. However, there was a significant decrease of MSP value (82±7 mm Hg) in MKT-H2 group compared to Control-group (93±9mmHg) andMCT-Control group (95±7mmHg) (p<0.05). Histological analysis showed that the number of MCs (including those containing tryptases) in the MCT-H2 group was on average 50% lower than in the MCT-Control group (p<0.05). CONCLUSIONS: Breathing air containing 4% hydrogen for 3 weeks after injection of MCT had no effect on the degree of MCT-PH development, but resulted in a decrease in MSP, which was accompanied by a decrease in the signs of lungs inflammation.

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