Program and book of abstracts 1st conference

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Medvedev Oleg

Chairman Department of Pharmacology since 1992, Dean-organizer (1992 2000) of the Faculty of Fundamental Medicine, Lomonosov Moscow State University. Part time professor at the Bauman Moscow Technical University. Research interests include effects of antioxidants on the cardiovascular and endocrine systems, analysis of the effects of exogenous and endogenous (from the gut microbiota) hydrogen. Personalized medicine. Published more than 220 papers. PERSONALIZED APPROACH TO THE USE OF EXTERNAL MOLECULAR HYDROGEN IN MEDICINE O. Medvedev 1 , A. Ivanova 1 , M. Belousova 1 , O. Obolenskaya 1 , T. Kuropatkina 1 , I. Shirokov 2 1 FACUTY OF FUNDAMENTAL MEDICINE, LOMONOSOV MOSCOW STATE UNIVERSITY, MOSCOW, RUSSIA 2 FACULTY OF BIOMEDICAL ENGINEERING, BAUMAN MOSCOW TECHNICAL UNIVERSITY, MOSCOW, RUSSIA BACKGROUND: There are two options to increase the concentration of molecular hydrogen (H 2 ) in the blood: 1- stimulation of the production of endogenous H 2 by the gut microbiota; 2– to take exogenous H 2 by drinking hydrogen-rich water or by the inhalation. It is known that about 50% of humans produce not only H 2 but a methane (CH 4 ) as well. Whether high CH 4 - producers can produce more H 2 remains unknown. OBJECTIVES: The goal was to evaluate of the effectiveness of H 2 -production stimulation in the rat models of high and low CH 4 -producers. METHODS: Experiments were carried out on 2 groups -25 low CH4 -producing rats were obtained from “Puschino” nursery and 25 high CH 4 -producing rats were obtained from "Stolbovaya" nursery. The samples of breath air from individual fasting rats were collected using modified rat nose-only Allay restrainer and analyzed for H 2 and CH 4 by GC (Trilyzer mBA-3000, Japan). For intragastric carbohydrate (CH) load: lactulose (2.0 g/ kg), PHGG (Optifiber®) (4.0 g/kg) and inulin (4.0 g/kg) were used. Samples of breath were collected before and 2, 4, 6 and 8 h after carbohydrate load. Composition of microbiome was analyzed in feces by 16S-rRNA method. RESULTS: CHs used evoked different rise in breath H 2 in low CH 4 -producing rats, the highest response was to lactulose and the lowest - to inulin. We did not observe differences in CH4 production in responses to CH load. In group of high CH 4 -producers CH load evoked 12-times higher rise in the level of CH 4 compared with H 2 (p<0.0001). Inulin evoked the weakest response. Beta diversity of microorganisms in high CH 4 -producers was much higher vs group of low CH 4 -producers. CONCLUSIONS: We suggest to perform preliminary H2/CH4 breath tests to screen the high CH4-producers who might be more responsive to administration of exogenous H 2 .

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