Program and book of abstracts 1st conference

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Todorovic Nikola

Nikola Todorović is currently working as a teaching assistant at the Faculty of Sport and Physical Education, University of Novi Sad. He is also a Ph.D. student and lab technician investigating various interventions' effects on biomarkers of energy metabolism and health-related lifestyle behaviors in clinical and non-clinical populations. The topic of his doctoral dissertation is the examination of the effect of molecular hydrogen on cellular bioenergetics. Nikola Todorović, M.sc, has experience implementing scientific knowledge in practical work with various populations, from clinical patients to experience athletes.

GENDER-SPECIFIC MOLECULAR HYDROGEN AND CAFFEINE SUPPLEMENTATION ON ALERTNESS AND BRAIN METABOLISM IN SLEEP-DEPRIVED HEALTHY ADULTS N. Todorovic 1 , D. Javorac 1 , V. Stajer 1 , S.M. Ostojic 1, 2,3 1 Applied Bioenergetics Lab, Faculty of Sport and PE, University of Novi Sad, Novi Sad, Serbia, 2 Faculty of Health Sciences, University of Pecs, Pecs, Hungary, 3 Department of Nutrition and Public Health, University of Agder, Norway. BACKGROUND: In the past two decades, molecular hydrogen (H2) has been clinically established to provide antioxidant and anti-inflammatory effects. However, the evidence regarding H2 effects on brain metabolism and executive function is still scarce. OBJECTIVE: The present study aimed to assess the acute effects of drinking a single dose of hydrogen-rich water (HRW) and compare it with caffeine, HRW plus caffeine, and control water for brain metabolism, alertness and executive function, and determine different gender responses in sleep-deprived participants. METHODS: Sixteen healthy young adults (8 male and 8 females; age 24.0 ± 3.5 years, body mass index 23.5 ± 2.4 cm) voluntarily participated in this randomized, controlled, cross over trial. After 24-hr sleep deprivation period, outcomes were assessed at baseline (pre intervention) and 15-min after drinking the beverage. HRWtablets were provided by HRW Natural Health Products Inc. (New Westminster, BC, Canada). The outcomes included, attention network test (ANT), trail-making test (TMT), and symbol digit modalities test (SDMT) as indicators of alertness and executive function. Magnetic resonance imaging and spectroscopy were performed to assess the choline-to-creatine ratio in different brain regions. RESULTS: For male participants, a significant time X treatment was found for reaction time and SDMT outcomes (p <.05) for both HRW and caffeine, while only caffeine influenced the accuracy of the ANT test. Female participants had significant improvement in TMT duration and SMDT outcomes but only for HRW and HRW plus caffeine intervention (p <.05). Moreover, HRW and caffeine significantly increased the choline-to-creatine ratio in several brain regions, while HRW also affected brain metabolism in the paracentral brain, with no differences between genders. RESPONSES AFTER

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