Program and book of abstracts 1st conference

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Cervena Tereza

Works as a junior toxicologist at the Department of Nanotoxicology and Molecular epidemiology (Institute of Experimental Medicine CAS) since 2014. Currently working on her PhD topic “Application of 3D tissue models of lung epithelium to study toxic effects of air pollutants and engineerednanoparticles” under dr. Rössner Jr. supervision. Her expertise is in air-liquid exposuremodels and exposure systems with a focus on engine emissions and nanoparticles. In 2019, the research team of dr. Rössner, in collaborationwithCVUT andCZU, developed an air-liquid exposure system and patented it (Toxicological incubator for aerosol exposure of cell cultures, CZ308427B6). To focus on her expertise, in 2020, she completed an internship at Swansea University Medical School (Wales, UK) under the supervision of Prof. Shareen Doak. Since 2022 she works on the project “The impact of real-world ambient air pollution exposure on human lung and olfactory cells grown at the air-liquid interface”, which is a follow-up of the patented exposure system. EFFECT OF MOLECULAR HYDROGEN ON BIOLOGICAL PROCESSES OF MUCILAIRTM 3D TISSUE CULTURES EXPOSED TO TOTAL ENGINE EMISSIONS Tereza Cervena 1 , Jan Topinka 2 , Hasmik Margaryan 2 , Anezka Vimrova 1 , Michal Vojtisek Lom 3 , Pavel Rossner Jr. 1 1 Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, 142 00, Prague, Czech Republic 2 Department of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine CAS, 142 00, Prague, Czech Republic 3 Institute of Mechatronics and Technical Informatics (MTI), Technical University of Liberec, Liberec, Czech Republic The antioxidant potential of molecular hydrogen (H2) and its possible positive effect on oxidative damage is a subject of interest in both the private and public sectors. In our study, we focused on the effect of H2 applied in an air-liquid environment on the MucilAirTM 3D lung tissue model, which was exposed to engine emissions from a gasoline engine. 3D models from two different donors were exposed to a 3% H2 mixture pre-exposure, post-exposure, or both pre- and post exposure to total emissions. Selected parameters of cytotoxicity (LDH), cell culture integrity (TEER), oxidative stress, antioxidant response and inflammationmarkers (mRNAexpressionand proteins) were measured in the cell culture medium and cell lysates. Cytotoxicity results confirm the suitability of the exposure scheme for repeated tests in all cases of H2 use (pre-exposure, post exposure, or both pre- and post-exposure to total emissions). The total toxicity measured in the cell culture medium did not exceed 10% and transmembrane potential measurements confirm these results. Western blot analysis of proteins showed different expressions of catalase (CAT) between donors with no H2 exposure effect. Likewise, no difference was observed in the case of the antioxidant enzyme superoxide dismutase (SOD). In contrast, however, an increasedpresence of the enzyme glutathione peroxidase (GPx) was detected in samples that were exposed to 3%H2 before exposure to total engine emissions, suggesting a positive antioxidant effect of H2. This trend was not observed in control samples exposed to filtered air. The results of the expression of selected cytokines and antioxidant enzymes are in the process of preparation.

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