Program and book of abstracts 1st conference

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Sumbalova Zuzana

RNDr. Zuzana Sumbalová, PhD. is a senior researcher working at Faculty of Medicine, Comenius University in Bratislava. She is an expert in the field of mitochondrial respirometry and bioenergetics. Her current focus is on mitochondrial respiration of human platelets in diseases such as rheumatoid arthritis, chronic kidney diseases, COVID-19. The collaborations include evaluation of mitochondrial function in cell cultures, permeabilized muscle tissue, mitochondria isolated from rat brain, skeletal muscle, heart. Within a long-term collaboration with Oroboros Instruments (Austria), she participated in the development of methods for high-resolution respirometry, which are used worldwide. Dr. Sumbalová is author and coauthor of 49 scientific publications registered in database Scopus with 518 citations. She is a member of Mitochondrial Physiology Society, was awarded by the International Society for Neurochemistry for the best poster (Lausanne 2006). She was a Scientific representative of grant holder for COST Action CA15203 MitoEAGLE and a substitute for Austria in the management committee (2017 – 2018). EFFECTS OF MOLECULAR HYDROGEN ON ANTIOXIDANT CONTENT AND MITOCHONDRIA FUNCTION IN PATIENTS WITH NON-ALCOHOLIC FATTY LIVER DISEASE Z. Sumbalová 1 , J. Kucharská 1 , Z. Rausová 1 ,M. Szántová 2 , V.Mojto 2 , B. Kura 3 , A. Gvozdjáková 1 , J. Slezák 3 1 Pharmacobiochemical Laboratory of 3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia, 2 3rd Department of Internal Medicine, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia, 3 Institute for Heart Research, Center of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia. BACKGROUND: Molecular hydrogen has been recognized as a novel medical gas with antioxidant and anti-inflammatory effects. Non-alcoholic fatty liver disease (NAFLD) is a liver pathology with increased fat accumulation in liver tissue caused by factors other than alcohol consumption. Platelet mitochondrial function is considered to reflect systemic mitochondrial health. OBJECTIVES: In this study, we followed the effect of supplementary therapy with hydrogen rich water (HRW) on lipophilic antioxidants content and platelet mitochondrial function of patients with NAFLD. METHODS: 30 patients with NAFLD and 15 healthy volunteers were included into the study. 17 patients (H2 group) drunk water 3x330 mL/day with tablets producing HRW (>4 mg/L H2) for 60 days and 13 patients (P group) drunk water with placebo tablets producing CO 2 . The concentration of α-tocopherol, β-carotene and CoQ10-TOTAL was determined by HPLC method, the parameter of oxidative stress, TBARS was determined by spectrophotometric method. Mitochondrial respiration in platelets isolated from whole blood was determined by high-resolution respirometry.

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