Program and book of abstracts 1st conference

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Hyspler Radomir

Is deputy of Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Czech Republic. His research interests are dihydrogen antioxidative effects as well as other metabolites originating in human large bowel, such as uraemic toxin indoxyl sulfate. He has published over 180 scientific articles (H-index 18). He is teaching Clinical Chemistry in Medical fakulty in Hradec Kralove – Charles University, Prague.

THE ROLE OF MOLECULAR HYDROGEN AS HIGHLY BIOAVAILABLE ANTIOXIDANT

R. Hyšpler 1 , A. Tichá 1 , L. Pavlíková 1 , Z. Zadák 1 1 Dpt. of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Sokolská 581, Hradec Králové 500 05, Czech Republic, rhyspler@lfhk.cuni.cz BACKGROUND: Molecular hydrogen (H 2 ) antioxidant properties were described in 2007 and years of intensive research followed. Hydrogen combines numerous advantageous properties not seen together in other antioxidants, such as high diffusion coefficient and bioavailability (even the ability to quickly reach mitochondria), non-toxicity, presence in both hydrophilic and lipophilic environments in tissue. Hydrogen is naturally present in the splanchnic area as it originates from fermentation processes in the large bowel. OBJECTIVES: Molecular hydrogen quantitative metabolic parameters in vivo are still lacking, as is an appropriate method for determination of hydrogen effectivity in the mammalian organism under various conditions. METHODS: Intraperitoneally-applied deuterium gas was used as a metabolic tracer and deuterium enrichment was determined in the body water pool in rats. Another study performed used domestic pigs, tested group underwent resection left lobe liver resection with inhaled hydrogen during general anesthesia, and control group underwent the same procedure using conventional general anesthesia. Samples from the ischemic and non-ischemic halves of the liver were then removed for histological and biochemical examinations. RESULTS: A significant oxidation of about 10% of the applied deuterium dose was found under physiological conditions in rats, proving its antioxidant properties. Hypoxia or endotoxin application did not exert any effect, whilst pure oxygen inhalation reduced deuterium oxidation. In the model of liver resection, the average Suzuki score for left lobes was 1.000±0.274 (left × hydrogen) in the intervention group, compared to 1.667±0.274 in the control group (left × no hydrogen), which corresponds to a difference in average values of 66.7%. In right (non-ischemic) lobes, the mean Suzuki score was 0.167±0.274 (right × hydrogen) in the intervention group versus 0.833±0.274 in the control group (right × no hydrogen). CONCLUSIONS: The measurable oxidation of stable hydrogen isotope was observed in rats, confirming its antioxidant properties in vivo. In surgical experimental study, we showed that inhaled hydrogen during major liver resection unquestionably reduced the level of oxidative stress associated with ischemia-reperfusion damage. This work was supported by MH CZ – DRO (UHHK, 00179906)

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