Program and book of abstracts 1st conference

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Hassanian Seyed Mahdi

Dr. Seyed Mahdi Hassanian is an associate Professor in Clinical Biochemistry. He is a faculty member in Mashhad University of Medical Sciences. His research interest is on finding novel anti-inflammatory molecules including hydrogen, in decreasing adhesion band formation post-injuries. During these years our team has published more than 170 manuscripts which are available at: https:// pubmed.ncbi.nlm.nih.gov/?term=hassanian+sm&sort=date. Dr. Hasssanian Received honorary letter, young inventors and innovators of Iran, in 2008. He received the Gold medal and Diploma in the “37th International Exhibition of Inventions” Geneva, Switzerland. 2009. He is also the recipient of the Gold medal and Diploma as the best young scientist (Under 35 years old) in the country, in the “24th International Razi Festival” Tehran, Iran. 2019. HYDROGEN-RICH WATER REDUCES INFLAMMATORY RESPONSES IN MICE DURING ATROPHY S. M. Hassanian 1 , F. Asgharzadeh 2 , G. Khalili 3 , M. Ryzhikov 4 , 1 Department of Clinical Biochemistry, Mashhad University of Medical Sciences, Mashhad, Iran, 2 Department of Medical Physiology, Mashhad University of Medical Sciences, Mashhad, Iran, 3 Department of Human Genetics, Mashhad University of Medical Sciences, Mashhad, Iran, 4 Department of Biochemistry, Saint Louis University, MO, USA. BACKGROUND: Hydrogen-rich water is a safe and effective way to introduce hydrogen into muscle tissues.Muscular Atrophy due to injury is a common complication of degenerative diseases. OBJECTIVE: This study aimed to determine anti-inflammatory mechanisms of Hydrogen-rich water and its therapeutic potentials in decreasing inflammation during skeletal muscle atrophy. METHOD: Mice were subjected to unilateral hind limb immobilization for a week and treated in the presence and absence of hydrogen-rich water. At the end of the experiment mice were sacrificed and hind limb muscle was collected. Muscle tissue was sectioned and stained with Hematoxylin andEosin staining. Fibrosiswas evaluated in tissue sections byMasson’s Trichrome staining. Total RNA and protein were extracted from skeletal muscle and expression levels of inflammatory markers were evaluated using Real-Time PCR and ELISA respectively. RESULTS: We found that hydrogen-rich water enhances myofiber diameter in skeletal muscle tissues significantly. Interestingly, hydrogen-rich water could attenuate atrophy induced fibrosis of skeletal tissues as visualized by tissue staining. Moreover, protein level of Interleukin-6 (IL-6) was decreased in mice treated with hydrogen-rich water. Our results showed that mRNA levels of inflammatory markers including Tissue necrosis factor alpha (TNF-α), IL-6, were down-regulated in the presence of hydrogen-rich water. CONCLUSION: These results showed anti-inflammatory mechanisms of hydrogen-rich water and support the hypothesis that this safe, easily-accessible, and cost-effective treatment can be a novel candidate for further investigation in inflammatory degenerative diseases. This work was supported by grants awarded by National Institute for Medical Research Development to S. M. H.

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