Program and book of abstracts 1st conference

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Song Guohua

Guohua Song, Young Taishan Scholars Scientist of Shandong Province, professor at Shandong First Medical University, supervisor for Ph.D postgraduates, Deputy director in School of BasicMedical Sciences, Shandong FirstMedical University. Dr. Guohua Song received her Ph.D of Medical Science fromYonsei University, South Korea. Her research focuses on lipid and lipoprotein metabolism and the application of molecular hydrogen, an emerging anti-oxidative gas. Her work has been supported by the National Science Foundation of China for 3 times, and also by the Science Foundation of Shandong Province for 2 times. She has published more than 30 SCI papers, including J Clin Endocrinol Metab, Free Radi

Bio Med, J Lipid Res, BBA-MOL CELL BIOL L, and Atherosclerosis. Besides, she was appointed as members of the Standing Committee of the Molecular Hydrogen Biomedicine, members of the Young Scholar Committee of Biochemical and Molecular Pharmacology, members of the Specialist Expert Committee of Molecular Hydrogen Biomedical Special fund for development. In addition, she was awarded "Science & Technology Award for Young Talents" by Shandong Province and she is one of the main candidate to receive the Award of the science and technology progress in Shandong Province. In recent years, she was invited to attend the Academic conferences at home and abroad and provide the oral presentations as the invited speaker for many times. HYDROGEN REDUCES NEUTROPHIL EXTRACELLULAR TRAPS (NETS) FORMATION IN ATHEROSCLEROSIS Tingting Qiu, Guohua Song* School of Basic Medical Sciences and The Second Affiliated Hospital of Shandong First Medical University & Shandong Academy of Medical Science, Taian 271000, China *Correspondence to Guohua Song (email: ghsong@sdfmu.edu.cn) Neutrophil extracellular traps (NETs) were discovered as one of the defense mechanisms of neutrophils in response to various infection. NETs were found in human and mouse plaques, and inmice, they are associated with increased atherosclerosis progression. We have previously found that hydrogen can inhibit the inflammatory response of atherosclerotic plaques and delay the occurrence and development of atherosclerosis. Here, we sought to explore the effects of hydrogen on NETs formation during atherosclerosis. Eight-week-old male apoE-/- mice fed a high-fat diet were treated with H2-O2 mixture (67% hydrogen and 33% oxygen) for 2 h/day for 8 weeks. NETs formation was evaluated by immunofluorescence staining and myeloperoxidase (MPO)-DNA complex ELISA. The hematoxylin and eosin (H&E) and oil-red O staining were performed to determine the lipid area and plaque size. For in vitro experiment, neutrophils isolated fromhealthy volunteers were used to determine the effects of hydrogen on NETs formation. The expressions of myeloperoxidase (MPO), elastase (NE), p-ERK, ERK, p-p38 and p38 in neutrophils were detected by Western blotting after PMA-stimulation for 3 hours. Results showed that administration of hydrogen not only reduced atherosclerotic plaque area, but also reduced the formation of NETs both in vivo and in vitro. The up-regulated expressions of MPO, NE, p-ERK and p-p38 by PMA in isolated neutrophils were significantly decreased after hydrogen treatment. Our findings indicate that hydrogen can reduce the formation of NETs in atherosclerosis via down regulating the expressions of NE and MPO, which might provide a novel scientific basis for the understanding of the effects of hydrogen on atherosclerosis progression.

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