Program and book of abstracts 1st conference

1 st Conference of European Academy for Molecular Hydrogen Research in Biomedicine “Hydrogen for Biomedicine“

Kura Branislav

Is an independent researcher at the Institute for Heart Research, Center of Experimental Medicine of SAS. He is a young scientist under 35 years of age with a focus on research into themolecular biology of heart diseases. He has published morethan25worksinthisfield.Duringhisstudieshewasawarded: -TravelAward Competition in ISAMcongress, Yonago, Japan 2015, - Travel AwardCompetition in IASC-NASCongress, Sherbrooke,Canada in2016, -TravelAwardCompetition in IACS-ES Congress, Marseille, France in 2016, - Early Carrier Investigators Competition Award at IACS-ES congress, Smolenice, Slovak Republic in 2018. Since 2018 he has also received a grant from Štefan Schwartz awarded by the Slovak Academy of Sciences. In 2018 he completed an internship at the St. Boniface Research Center inWinnipeg, Canada. THERAPEUTIC EFFECT OF MOLECULAR HYDROGEN ON THE HEART DURING IST SIMULATED TRANSPLANTATION B. Kura 1 , B. Kalocayova 1 , V. Hudec 2 , M. Ondrusek 2 , I. Gasparovic 2 , R. Sramaty 2 , J. Luptak 2 , P. Pavelkova 1 , M. Hulman 2 , J. Slezak 1 1 Centre of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovak Republic 2 Department of Cardiac Surgery, Faculty of Medicine, National Institute of Cardiovascular Diseases, Comenius University, Bratislava, Slovak Republic BACKGROUND: Currently, the heart transplantation (HT) is a common procedure for patients with failing hearts. However, after the donor heart is implanted in the recipient, the so-called ischemic-reperfusion damage occurs, leading to mitochondrial dysfunction, inflammation, and overproduction of reactive oxygen species. In the last 15 years, molecular hydrogen (H2) was widely studied for its antioxidative, anti-inflammatory, and anticytotoxic effects in many experimental models, including ischemia-reperfusion injury. OBJECTIVES:The aim of this study was to examine the effect of H2 on the heart of pigs (female, 4 months old) who underwent a simulated HT (sHT). METHODS: sHT consisted of occluding venae cavae and pulmonary veins, cross clamping of ascending aorta, and connection to ECC. Cold crystalloid cardioplegia was administered for three hours. Then the aortic clamp was released, and heart was rewarmed. After 60 minutes of spontaneous reperfusion, the experiment was terminated. H2 was administered in a gas form during blood oxygenation (50% O2, 3% H2). In this study, levels and activities of oxidative and heart damage markers, and the expression of microRNAs (miRNAs) were measured from blood plasma and left ventricle tissues. RESULTS: sHTsignificantly increasedactivitiesandamountsof endogenicantioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase), molecules of oxidative stress damage (uric acid, malondialdehyde, lactate dehydrogenase, 8-hydroxy-2-deoxyguanosine), and heart damage (troponin T, creatine kinase, myoglobin). H2 treatment significantly improved all parameters almost up to control levels. The sHT also caused significant changes in the levels of selected miRNAs where H2 treatment had normalization effects either. CONCLUSIONS: Addition of H2 during HT could be a new potential therapeutic strategy for minimalizing the negative effect of ischemia/reperfusion injury, leading to better recovery of patients. Funding: APVV-19-0317, 2018/7838:1-26C0, 2019/4-CEMSAV-1, VEGA 2/0092/22 and 2/0148/22.

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