Program and book of abstracts 1st conference

October 17 th – 20 th , 2022, Congress Centre of Slovak Academy of Sciences, Smolenice castle, Slovakia

Khazaei Majid

Dr. Majid Khazaei is a professor of Medical Physiology in Mashhad University of Medical Sciences, Mashhad, Iran. He is a faculty member working on protective responses of anti-inflammatory molecules such as H2 in human diseases including atrophy, colitis, and colon cancer. More than 225 manuscripts have been published by Prof. Khazaei. His manuscripts can be accessed in the link below: https://pubmed.ncbi.nlm.nih.gov/?term=majid+khazaei&sort=date

HYDROGEN-RICH WATER COULD IMPROVE MUSCLE ATROPHY IN A HINDLIMB IMMOBILIZATION MODEL IN A MICE DURING ATROPHY AND RECOVERY PHASES M. Khazaei 1 , E. Nazari 1 , N. Khalili 1 1 Department of Medical Physiology, Mashhad University of Medical Sciences, Mashhad, Iran BACKGROUND: Muscular Atrophy is a serious concern found in a number of human diseases. Hydrogen-richwater is a potentially novel, therapeutic strategy for attenuating clinical symptoms in inflammatory-associated diseases including muscle atrophy. Objective: In this study we investigated therapeutic effects of hydrogen-rich water (HRW) on muscle performance and recovery in a muscular atrophy mice model. METHOD: Skeletal muscle atrophy was induced by 7-days of hind limb immobilization. BALB/c mice were divided into the Sham, no-treated, and H2-treated atrophy groups (n=12). We administered 200 µL HRW orally by a fast-dissolving hydrogen tablet in drinking water. Muscle weight, grip strength, and the cross-sectional area of muscle fibers, were compared between groups. In those exposed to recovery, the splint was removed after 7-days of hind limb immobilization, and then the sign of muscle regeneration was assessed at the end of the recovery period. RESULTS: Our results showed that administration of H2 significantly prevented atrophy of Gastrocnemius muscle fibers, as compared to the no-treated group. Moreover, Gastrocnemius muscleweight andmuscle to total bodyweight were potently improved in theH2-treated group. We also showed that H2 improved four limb grip strength, and increased cross-sectional area of muscle fibers during both atrophy and recovery phases. Furthermore, compared to the no treated group, circulating level of Troponin-I, as an atrophy biomarker, decreased remarkably in the H2-treated group. CONCLUSION: Our results showed that H2-rich water is a promising therapeutic candidate for regulating muscle regrowth during the atrophy and recovery phases. Nevertheless, further clinical studies should be conducted to study all aspects of this novel treatment in patient. Key words: Hydrogen, H2-rich water, Muscle atrophy, Muscle recovery This work was supported by grants awarded by Mashhad University of Medical Sciences (Grant No. 961077) to M. K.

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