Book of Abstracts - New Frontiers 2022

Abstracts of poster presentations

HMGB1 AS A POTENTIAL TARGET FOR TREATMENT AFTER EXPERIMENTAL MYOCARDIAL INFARCTION

M. Cebova, A. Barta, O. Pechanova

Institute of Normal and Pathological Physiology, Centre of Experimental Medicine SAS, Bratislava, Slovakia

Myocardial infarction (MI) remains a leading cause of morbidity and mortality among all cardiovascular diseases over the world. High mobility group box 1 (HMGB1) is a DNA-binding protein with multiple cardioprotective effects. Besides its nuclear role, HMGB1 released during heart ischemia participates in interaction including the production of proinflammatory cytokines. The aim of the study was to evaluate the effects of anti-HMGB1 protein on biochemical and morphological parameters after experimentally induced MI in 12-week-old male WKY rats. In vivo model of experimental MI was induced by ligation of the left descending coronary artery and lasted 20 min. Prior to reperfusion anti-HMGB1 protein was administrated i.v. 7 days after MI, nitric oxide synthase (NOS) activity was determined by conversion of 3[H] Arginine to 3[H] Citrulline in the aorta and ischemic, border and non-ischemic region of the heart. NFҡB, iNOS and eNOS expression was deter mined by Western blot. For morphological parameters, the hearts were excised and used for TTC-staining procedure. Cytokine levels were investigated using the Bio Plex Pro Cytokine kit in the plasma. Concentration of conjugated dienes was measured spectrophotometrically in the heart. Anti-HMGB1 protein increased NOS activity in both ischemic and border heart zone, as well as in the aorta, on the other hand NOS activity was not changed in non-ischemic part of the heart. The same pattern was found in eNOS expression level. Anti-HMGB- 1 protein administration decreased iNOS and NFҡB expression in the ischemic zone as well as TNF-alpha and IL-6 level in plasma. Moreover, anti-HMGB-1 protein decreased the level of conjugated dienes in the heart. Simultaneously, anti HMGB1 protein decreased ischemic part as well as border region of the heart. Considering the results by using a rat model of experimentally induced MI, HMGB1 protein is a promising molecule for reduction the negative effects of the myocardium infarction, for the amelioration of inflammation, as well as a promising approach for treatment of cardiovascular diseases.

Keywords: myocardial infarction, HMGB-1 protein, nitric oxide

Funding:VEGA 2/0132/20

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