Book of Abstracts - New Frontiers 2022

Abstracts of oral presentations

THE EFFECT OF IRON OXIDE NANOPARTICLES ON VASCULAR FUNCTION OF THE FEMORAL ARTERY OF NORMOTENSIVE RATS

S. Liskova 1,2 , P. Balis 1 , A. Micurova 1 , I. Bernatova 1

1 Institute of Normal and Pathological Physiology, Centre of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia; 2 Institute of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Comenius University, Bratislava, Slovakia Superparamagnetic iron oxide nanoparticles have the potential to be used in various biomedical applications including drug delivery and magnetic hyperthermia for the treatment of diverse carcinomas. The iron oxide nanoparticles are administered intravenously, thus their administration may influence the vascular tone regulation. The aim of our study was to investigate the effect of polyethylene glycol-coated Fe3O4 nanoparticles (IONs) on vascular functions of femoral arteries in Wistar-Kyoto rats. We investigated control and ION-treated rats (single dose 1 mg Fe/kg i.v., suspended in saline, 30 nm core size, ~51 nm hydrodynamic size). Vascular function was determined 100 min after ION infusion. Mean arterial pressure (MAP) and heart rate (HR) were not significantly different at the beginning of the experiment. The i.v. administration of IONs has not influenced MAP and HR at the end of experiment. KCl- and serotonin induced contractions were not different between control and ION-treated group. Maximal acetylcholine (ACh)-induced and sodium nitroprusside (SNP)-induced relaxations in the femoral arteries did not differ among the groups. After NOS inhibition with Nω -nitro-L-arginine methyl ester (L-NAME) the serotonin induced contraction was increased in control group, but not in ION-treated group. The L-NAME-sensitive component of ACh-induced relaxation was enhanced in ION-treated group suggesting enhanced production of NO in the femoral artery after ION application. On the other hand, the sensitivity of vascular smooth muscle cells to sodium nitroprusside was decreased in ION-treated group showing an adaptation of the vascular smooth muscle cells of femoral arteries to enhanced NO bioavailability. In conclusion, our results showed that single i.v. administration of IONs altered vascular function by preventing the increase of serotonin-induced contraction after NOS inhibition and by increasing the L-NAME-sensitive component of ACh-induced relaxation indicating augmented NO release from the endothelium in femoral arteries.

Keywords: iron oxide nanoparticles, femoral artery, endothelium

Funding:This study was supported by the grants No. VEGA 2/0157/21 and APVV-16-0263.

43